|Title:||Interlobar IL-8 variation in pre-school children with CF. Findings of the Study to Evaluate the Additional Gains of Upper and Lower Lobe Sampling in Children with CF (SEAGULLS).|
|Author(s):||Butler D1,3, Montague T2, O’Sullivan N2, Cox D1,2, McNally P1,2,3.|
|Institution:||1. National Children’s Research Centre, Crumlin, Dublin, Ireland. 2. Children’s Health Ireland at Crumlin, Crumlin, Dublin, Ireland. 3. RCSI Department of Paediatrics, RCSI, Dublin, Ireland.|
|Poster:||Click to view poster|
|Abstract:||Six lobe Bronchoalveolar lavage (BAL) during bronchoscopy in children has been reported to be safe and confer a higher microbiological yield. Regional variability in CF lung disease is well described clinically but the etiology remains incompletely understood. The aim of our study is to assess a standardised six lobe BAL collection technique using 2x1ml/kg/lobe and to evaluate the factors contributing to regional variability in infection and inflammation. Samples were processed into individual lobar, two (RML & lingula) and six lobe pooled aliquots. In this interim analysis, IL-8 was measured in BAL supernatant using a commercially available BioLegend ELISA kit.|
Samples from 18 subjects were included with mean age 3.9yrs (range 1.46-6.18yrs) with equal gender distribution. Pooled two and six lobe samples did not demonstrate significantly different IL-8 levels, though there was significantly higher variation in lavaged 2 lobe pooled samples compared to 6 lobe pooled samples (CoV P=0.05). Across each lobe there is evidence of increasing IL-8 as children age. There evidence of higher IL-8 levels in the same lobe based on gender.
This interim analysis demonstrated variable IL-8 concentrations across anatomically separated lung regions. Further analysis with larger numbers is underway to assess this lobar variability more comprehensively. With ongoing analysis in the context or NE, pepsin and microbiome.