|Title:||Proteomic Analysis Of The Nanoparticle Biomolecular Corona Formed In The Plasma Of Lung Cancer Patients And Healthy Controls|
|Author(s):||I Marinescu ND Trinh MP Keane M Monopoli C McCarthy|
|Institution:||UCD and St Vincent's University Hospital, RCSI|
|Poster:||Click to view poster|
|Category:||Lung Cancer and Bronchoscopy|
|Abstract:||A challenge posed by lung cancer biomarker screening is that target proteins have low molecular weights and are too difficult to isolate by standard mass spectrometry (MS) alone. Using nanoparticles (NPs) and the protein corona formed on their surface to isolate molecules of interest may be a more efficient way to detect low-abundance proteins related to malignancy. In this study, we characterize the protein corona formed on silica NPs incubated with human plasma from lung cancer patients and healthy volunteers. |
Samples were analysed using dodecyl sulphate polyacrylamide gel electrophoresis and MS. The raw data was imported into MaxQuant for label-free quantification and then analysed using the softwares Perseus and Cytoscape.
Gel densitometry analysis revealed two significantly different protein bands between lung cancer and controls. MS analysis identified five significantly down-regulated proteins in the lung cancer group. The most abundant proteins identified were apolipoprotein A-I, fibrinogen, albumin and proteins of the complement family.
The results of our analysis provide information on the surface constitution of NPs introduced into plasma and pave the way for a better understanding of how this may influence their interaction with the internal environment. The down-regulation of several proteins in the cancer samples suggests an altered molecular make-up in malignancy and may contribute to the future discovery and implementation of specific biomarkers for lung cancer screening.